Writing & Erasing O-GlcNAc from Target Proteins in the Cell

Time: 10:00 am
day: Day Two


  • Over 15% of the cellular proteome is modified by O-linked N-acetyl glucosamine (O-GlcNAc), a post-translational modification (PTM) that consists of a single sugar attached to serine or threonine residues of nuclear, cytosolic and mitochondrial proteins
  • Due to the ubiquitous nature of the modification, O-GlcNAc has been implicated in numerous biological processes, including immune response, cancer progression, neurodegenerative disease, and diabetes
  • We have now developed a cellular approach for writing and erasing O-GlcNAc from target proteins in the cell. The method uses a nanobody fused to an O-GlcNAc writer or eraser to control O-GlcNAc levels on the desired target protein